Spermatogonial stem cells: questions, models and perspectives.

Spermatogonial Stem Cells: Biology, Isolation, Culture, Characterization, and Practical Perspectives

Spermatogonial stem cells (SSCs) also known as germ stem cells (GSCs) are the basis of spermatogenesis process in the testis. Furthermore, they are also valuable cells with different applications in developmental biology, transgenesis technology, and clinic. Understanding the new findings related to the cell and molecular biology of SSCs and the methods for isolation and maintenance of these ce...

Comparison of Neonatal and Adult Mice-derived Sertoli Cells in Support of Expansion of Mouse Spermatogonial Stem Cells In vitro

Combination of In Vivo Cryptorchid Testis and In Vitro Co- Culture System to Obtain High Purification and Proliferation of Mouse Spermatogonial Stem Cells

Background The present study was designed to evaluate the survival and proliferation of spermatogonial stem cells from cryptorchid mouse testis in co-culture system over a 3 weeks period. MaterialsAndMethods Sertoli and spermatogonial cells were isolated from bilateral cryptorchid mouse model testes. Isolated spermatogonial cells were co-cultured with Sertoli cells in minimal essential medium (...

Evaluation of the Effect of Follicle Stimulating Hormone (FSH) on Survival and Colonization of Caprine Spermatogonial Stem Cells during in Vitro Culture

Background and Objectives: Spermatogonial stem cells are specific cells that have the ability of self-renewal and differentiation. These cells play an essential role in maintaining spermatogenesis and fertility. In this regard, the present study was performed with the purpose of investigating the effect of different concentrations of follicle stimulating hormone (FSH) on in vitro colony formati...

The Effects of Plla Nanofiber Scaffold on Proliferation of Frozen-Thawed Neonate Mouse Spermatogonial Stem Cells

Purpose: To investigate of the effects of a poly L-lactic acid (PLLA) nanofiber scaffold on proliferation of frozen-thawed neonate mouse spermatogonial stem cells.Materials and Methods: Spermatogonial cells were isolated from neonatal 3-6-day-old NMRI mice testes by two steps enzymatic digestion and differential plating. The isolated spermatogonial cells were divided into four culture groups: 1...